Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin.
Identifieur interne : 003124 ( Main/Exploration ); précédent : 003123; suivant : 003125Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin.
Auteurs : Wuze Ren [République populaire de Chine] ; Xiuxia Qu ; Wendong Li ; Zhenggang Han ; Meng Yu ; Peng Zhou ; Shu-Yi Zhang ; Lin-Fa Wang ; Hongkui Deng ; Zhengli ShiSource :
- Journal of virology [ 1098-5514 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Assemblage viral, Cartographie d'interactions entre protéines, Cellules HeLa, Chimère (génétique), Chimère (physiologie), Chiroptera (virologie), Clonage moléculaire, Données de séquences moléculaires, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A (analyse), Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (métabolisme), Protéines virales (métabolisme), Pénétration virale, Rats, Récepteurs viraux (analyse), Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Souris, Séquence d'acides aminés, VIH (Virus de l'Immunodéficience Humaine) (génétique), Virus du SRAS (génétique), Virus du SRAS (physiologie), Viverridae (virologie).
- MESH :
- analyse : Peptidyl-Dipeptidase A, Récepteurs viraux.
- génétique : Chimère, Peptidyl-Dipeptidase A, Récepteurs viraux, VIH (Virus de l'Immunodéficience Humaine), Virus du SRAS.
- métabolisme : Peptidyl-Dipeptidase A, Protéines virales, Récepteurs viraux.
- physiologie : Chimère, Virus du SRAS.
- virologie : Chiroptera, Viverridae.
- Animaux, Assemblage viral, Cartographie d'interactions entre protéines, Cellules HeLa, Clonage moléculaire, Données de séquences moléculaires, Humains, Lignée cellulaire, Pénétration virale, Rats, Souris, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cell Line, Chimera (genetics), Chimera (physiology), Chiroptera (virology), Cloning, Molecular, HIV (genetics), HeLa Cells, Humans, Mice, Molecular Sequence Data, Peptidyl-Dipeptidase A (analysis), Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), Protein Interaction Mapping, Rats, Receptors, Virus (analysis), Receptors, Virus (genetics), Receptors, Virus (metabolism), SARS Virus (genetics), SARS Virus (physiology), Viral Proteins (metabolism), Virus Assembly, Virus Internalization, Viverridae (virology).
- MESH :
- chemical , analysis : Peptidyl-Dipeptidase A, Receptors, Virus.
- genetics : Chimera, HIV, Peptidyl-Dipeptidase A, Receptors, Virus, SARS Virus.
- chemical , metabolism : Peptidyl-Dipeptidase A, Receptors, Virus, Viral Proteins.
- physiology : Chimera, SARS Virus.
- virology : Chiroptera, Viverridae.
- Amino Acid Sequence, Animals, Cell Line, Cloning, Molecular, HeLa Cells, Humans, Mice, Molecular Sequence Data, Protein Interaction Mapping, Rats, Virus Assembly, Virus Internalization.
Abstract
Severe acute respiratory syndrome (SARS) is caused by the SARS-associated coronavirus (SARS-CoV), which uses angiotensin-converting enzyme 2 (ACE2) as its receptor for cell entry. A group of SARS-like CoVs (SL-CoVs) has been identified in horseshoe bats. SL-CoVs and SARS-CoVs share identical genome organizations and high sequence identities, with the main exception of the N terminus of the spike protein (S), known to be responsible for receptor binding in CoVs. In this study, we investigated the receptor usage of the SL-CoV S by combining a human immunodeficiency virus-based pseudovirus system with cell lines expressing the ACE2 molecules of human, civet, or horseshoe bat. In addition to full-length S of SL-CoV and SARS-CoV, a series of S chimeras was constructed by inserting different sequences of the SARS-CoV S into the SL-CoV S backbone. Several important observations were made from this study. First, the SL-CoV S was unable to use any of the three ACE2 molecules as its receptor. Second, the SARS-CoV S failed to enter cells expressing the bat ACE2. Third, the chimeric S covering the previously defined receptor-binding domain gained its ability to enter cells via human ACE2, albeit with different efficiencies for different constructs. Fourth, a minimal insert region (amino acids 310 to 518) was found to be sufficient to convert the SL-CoV S from non-ACE2 binding to human ACE2 binding, indicating that the SL-CoV S is largely compatible with SARS-CoV S protein both in structure and in function. The significance of these findings in relation to virus origin, virus recombination, and host switching is discussed.
DOI: 10.1128/JVI.01085-07
PubMed: 18077725
Affiliations:
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Le document en format XML
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Li</name></author><author><name sortKey="Han, Zhenggang" sort="Han, Zhenggang" uniqKey="Han Z" first="Zhenggang" last="Han">Zhenggang Han</name></author><author><name sortKey="Yu, Meng" sort="Yu, Meng" uniqKey="Yu M" first="Meng" last="Yu">Meng Yu</name></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name></author><author><name sortKey="Zhang, Shu Yi" sort="Zhang, Shu Yi" uniqKey="Zhang S" first="Shu-Yi" last="Zhang">Shu-Yi Zhang</name></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></author><author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name></author><author><name sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin.</title><author><name sortKey="Ren, Wuze" sort="Ren, Wuze" uniqKey="Ren W" first="Wuze" last="Ren">Wuze Ren</name><affiliation wicri:level="1"><nlm:affiliation>State key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071</wicri:regionArea><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name></author><author><name sortKey="Li, Wendong" sort="Li, Wendong" uniqKey="Li W" first="Wendong" last="Li">Wendong Li</name></author><author><name sortKey="Han, Zhenggang" sort="Han, Zhenggang" uniqKey="Han Z" first="Zhenggang" last="Han">Zhenggang Han</name></author><author><name sortKey="Yu, Meng" sort="Yu, Meng" uniqKey="Yu M" first="Meng" last="Yu">Meng Yu</name></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name></author><author><name sortKey="Zhang, Shu Yi" sort="Zhang, Shu Yi" uniqKey="Zhang S" first="Shu-Yi" last="Zhang">Shu-Yi Zhang</name></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></author><author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name></author><author><name sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Chimera (genetics)</term><term>Chimera (physiology)</term><term>Chiroptera (virology)</term><term>Cloning, Molecular</term><term>HIV (genetics)</term><term>HeLa Cells</term><term>Humans</term><term>Mice</term><term>Molecular Sequence Data</term><term>Peptidyl-Dipeptidase A (analysis)</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Protein Interaction Mapping</term><term>Rats</term><term>Receptors, Virus (analysis)</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Viral Proteins (metabolism)</term><term>Virus Assembly</term><term>Virus Internalization</term><term>Viverridae (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cartographie d'interactions entre protéines</term><term>Cellules HeLa</term><term>Chimère (génétique)</term><term>Chimère (physiologie)</term><term>Chiroptera (virologie)</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A (analyse)</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Protéines virales (métabolisme)</term><term>Pénétration virale</term><term>Rats</term><term>Récepteurs viraux (analyse)</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (métabolisme)</term><term>Souris</term><term>Séquence d'acides aminés</term><term>VIH (Virus de l'Immunodéficience Humaine) (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term><term>Viverridae (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chimera</term><term>HIV</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chimère</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term><term>VIH (Virus de l'Immunodéficience Humaine)</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term><term>Protéines virales</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Chimère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Chimera</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Viverridae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Viverridae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Cloning, Molecular</term><term>HeLa Cells</term><term>Humans</term><term>Mice</term><term>Molecular Sequence Data</term><term>Protein Interaction Mapping</term><term>Rats</term><term>Virus Assembly</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cartographie d'interactions entre protéines</term><term>Cellules HeLa</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Pénétration virale</term><term>Rats</term><term>Souris</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is caused by the SARS-associated coronavirus (SARS-CoV), which uses angiotensin-converting enzyme 2 (ACE2) as its receptor for cell entry. A group of SARS-like CoVs (SL-CoVs) has been identified in horseshoe bats. SL-CoVs and SARS-CoVs share identical genome organizations and high sequence identities, with the main exception of the N terminus of the spike protein (S), known to be responsible for receptor binding in CoVs. In this study, we investigated the receptor usage of the SL-CoV S by combining a human immunodeficiency virus-based pseudovirus system with cell lines expressing the ACE2 molecules of human, civet, or horseshoe bat. In addition to full-length S of SL-CoV and SARS-CoV, a series of S chimeras was constructed by inserting different sequences of the SARS-CoV S into the SL-CoV S backbone. Several important observations were made from this study. First, the SL-CoV S was unable to use any of the three ACE2 molecules as its receptor. Second, the SARS-CoV S failed to enter cells expressing the bat ACE2. Third, the chimeric S covering the previously defined receptor-binding domain gained its ability to enter cells via human ACE2, albeit with different efficiencies for different constructs. Fourth, a minimal insert region (amino acids 310 to 518) was found to be sufficient to convert the SL-CoV S from non-ACE2 binding to human ACE2 binding, indicating that the SL-CoV S is largely compatible with SARS-CoV S protein both in structure and in function. The significance of these findings in relation to virus origin, virus recombination, and host switching is discussed.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><name sortKey="Han, Zhenggang" sort="Han, Zhenggang" uniqKey="Han Z" first="Zhenggang" last="Han">Zhenggang Han</name><name sortKey="Li, Wendong" sort="Li, Wendong" uniqKey="Li W" first="Wendong" last="Li">Wendong Li</name><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name><name sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><name sortKey="Yu, Meng" sort="Yu, Meng" uniqKey="Yu M" first="Meng" last="Yu">Meng Yu</name><name sortKey="Zhang, Shu Yi" sort="Zhang, Shu Yi" uniqKey="Zhang S" first="Shu-Yi" last="Zhang">Shu-Yi Zhang</name><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Ren, Wuze" sort="Ren, Wuze" uniqKey="Ren W" first="Wuze" last="Ren">Wuze Ren</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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